Animal Farm Essays (479 words) - Literature, Fiction, British People

Animal Farm Based on viewing the movie Animal Farm and reading the novel of the same I have come to a base of centered agreements. I know that both of these stories differ dramatically in many ways and also the themes of each I believe are totally unlike one another. George Orwell wrote Animal Farm and I believe that this man purposes the same basic ideas as the movie as well as the novel even though he didn't write the screenplay for the movie. The main theme of the movie is highly more creative and more decisive. In the beginning the animals want nothing to do with humans nor do they try to be anything like them. They keep this principle in mind throughout the book but with some minor cracks along the way somehow this world without humans seems to be unbearable. At the end the animals find that somehow after all that has happened they too have been baptized with some of the human characteristics and act more and more like them in ways. I believe that the theme of the movie is that no matter how hard you try to change someone whether it is yourself, a fellow companion, or a stranger they will always bear the characteristics they had in the beginning. The theme of the novel is a bit more pessimistic than the audiovisual film. In the book we have the same basic idea of the animals not wanting to be anything like these humans but in the end a part of them corrupts causing a chain reaction. I think the theme of the novel is if you are trying to change society or the environment around you or even someone else you should not forget about the rest of us and concentrate what is going to improve what you have control over. But also don't forget about what your opinions are and what you can do to help not only yourself but others too. I think both the film and the novel have the same basic idea that the author and screenwriter is trying to get across to the viewers and readers. I think the point they are trying to get others to think about is that corruption happens to everyone. Some people do crazy things because they let certain things go to their head without thinking of the cause and effect it will take on everyone that has to deal with them. Power is the most brainwashing aspect of life. Someone with lots of power may do a good job at doing what they do but they do not have the absolute power to make everyone satisfied. In conclusion I believe these two different research projects have totally different themes but have one main idea that Orwell is trying to get the readers and viewers to see and decide for them selves. Science

Reform after the Revolution essays

Reform after the Revolution essays Although the United States gained independence after the Revolution from Britain, it was not without issues that needed to be addressed in order to promote a better way of life for the people. With the approaching new age came reformers that wanted to ensure that various improvements would occur in their country in order to enhance their lives. To the people, the only way to get the problems recognized on a national level was to organize social movements. Reformers sought answers to the problems within the prison system and also for the insane. Efforts were made to take the punishment out of the prison and use rehabilitation instead. Religious reformers opposed alcohol since they believed alcohol had effects on the work force and the family. Another effort of reform was the Antimasonry movement. Since it excluded women, it was believed by the reformers to be threatening to the family by encouraging men to use alcohol while neglecting their families. Since the Masons were made up of prominent middle and upper class men, it was believed to be a secret fraternity with antirepublican views. This was a very short-lived political movement. Although these issues did not accomplish the goals of the reformers they paved the way for much larger issues such as slavery. In the 1830s, along with a small minority of white reformers, William Lloyd Garrisons morals drove him to demand immediate and complete emancipation (Norton 242) of slavery. Those who advocated immediate emancipation saw slavery as a moral issue, a sin on the American nation. Although not all reformers believed in the immediate discontinuance of slavery, others tended to favor the gradual approach. The American Colonization Society had the idea of gradual abolition along with the resettling of the former slaves in Africa. Garrison, considered an immediatist, had strong opinions about the methods that should be used to ...

Fall 2010 November Elections Essay Example | Topics and Well Written Essays - 250 words

Fall 2010 November Elections - Essay Example That in itself might be a good thing. The flawed Healthcare bill is likely to be seriously amended by the Republicans. Obama tried to ram through a lot of bills that many Americans did not support. He also tried to spend his way out of the recession. This didnt work and people were very unhappy. With his stimulus bill he made a promise: Give me nearly $1 trillion to spend and I will give you more jobs. That didnt happen. The Republicans believe that a lot fewer of the solutions to the economic situation lie in the hands of government. They want the private sector to pick up the slack. The truth is that the outcome of the election may result in some deadlock but that is not necessarily a bad thing. The founding fathers were not big fans of government. They were happy to see government weakened which would allow people to make their own decisions in their own small towns. That is what we may see

EMPLOYEE RIGHTS, MANAGED CARE AND PATIENT SAFETY Essay - 3

EMPLOYEE RIGHTS, MANAGED CARE AND PATIENT SAFETY - Essay Example Managed care organizations, commonly referred as MCOs (Pozgar 2012) have been a key feature in this sector. According to the (Altenstettter &Bjorkman 2000) it has been seen that integration of both the insurer and provider functions been in the increase. Health Maintenance Organizations not only finances, but also ensure delivery of health services, which are comprehensive, to all the bonafide beneficiaries. The charges, under this model, are made on the employer for each subscriber. The fixed premium charge goes a long in giving the beneficiary rights of both being insured and provided medical care in case of ailments (Pozgar 2012). Subscriber should though note that, once in this system they can only get service from HMO contracted physicians. The HMO model has several forms which include staff and Independent practice association model. Under staff-model, an arrangement is made for the physician to proved services to the subscribers on full-time basis. The IPA- model has arrangeme nts made between the independent physicians and HMO to offer services on contract to the beneficiaries. Health care services can be bought from a group of providers who are carefully selected. Care in selection is emphasized so that the beneficiaries get the best treatment. This is accomplished through preferred provider organizations which are characterized by a well utilization management plan. Under this model, negotiation of payment rates and speeded payment terms are facilitated. In addition, the beneficiaries have freedom to choose services from other providers though at their own cost (Pozgar 2012). The major essence of this model is the negotiation of charges between the payer and panel providers whom the terms are on contract basis. The payer also selects a panel of providers whom are consulted by the enrollees in case of ailments or other medical necessities. This model has

Research database -fall incident Essay Example | Topics and Well Written Essays - 750 words

Research database -fall incident - Essay Example The falls also arise from the excessive use of psychoactive drug use and dementia. The abnormalities have resulted in the fall risks for a long time. However, the nursing role has helped in the cushioning of the vulnerable population against the fall incidences. The source is relevant to the subject of concern because it highlights the nursing care ensures that the impact of the fall is not severe and is attended to, in good time. The research studies on the proximate cause of the majority of the fall incidents reveals that the near the bed transfers account for most of the fall cases. According to Houser (2012), the nurses are advised to be careful during the transferring and mobilizing of the patients especially around the risky areas like the bed unit. A balance approach entails the creation of awareness of every person involved in the handling of the patients. The patient and family education immensely contributes to the reduction in the fall incidences. Post-fall management of the nursing department is also crucial because it helps in the recuperation process of the victims of the fall occurrences. Consequently, there is reduction j the injury cases with regard to the falls. According to Iyer and Aiken (2009), immediate response to the falls is important given that it saves lives. When a fall incident has occurred, the evaluation and monitoring of the victim is crucial. The evaluation includes the evaluation of the entire victim’s systems. Additionally, there should be a comprehensive description of the injuries. After the evaluation exercise is over, treatment of the victim follows. The process ensures that the real cause of the fall is established in order to take precautions in later undertakings. Additionally, there is a need for the nurse to carry out a complete falls assessment. The strategy helps in the overall analysis of the fall risks in the area. According to Haag-Heitman and George (2011), fall

Nonverbal Use Of Gestures

Nonverbal Use Of Gestures People express themselves nonverbally with the use of gestures (Archer 80). For instance, doing an A-OK sign by making a circle with ones index finger and thumb is an expression of approval to most Americans. When former U.S. President Richard Nixon deplaned in Caracas, Venezuela in 1956, he flashed two A-OK signs with his fingers to a group of demonstrators and caused a riot to erupt. The former President was later briefed that the meaning of the A-OK sign to Americans was equivalent to raising the middle finger to Venezuelans (Moran, Harris, and Moran 63). Despite being a crucial tool in effective communication, the meaning of nonverbal signals or gestures that people apply varies across different cultures. Hence, gestures are often misinterpreted. The silent signals attached to nonverbal communication are revealing. They can tell us motives, emotions, and feelings such as indecisions, honesty, joy, frustration, approval, anger, and many more (Goman). Being able to catch the meaning of the tiniest gestures that people make is important in everyday interpersonal communication and especially in the business world. Knowing what offends and what does not, what is proper and what is not, is an edge that individuals must be equipped with if they are to succeed in this competitive world (Stolte). Now that we live in a globalized world, diversity is a reality that all face and cultural differences in gestures need to be understood if we are to promote harmonious and effective communication. This paper presents cultural differences in gestures and nonverbal signals that have been studied or revealed in literature. Different Gestures, Different Meanings Subtle nonverbal practices or actions need to be understood because they have the possibility of offending others. The use of fingers, hands, and feet The thumbs-up sign which equates to Great! in the U.S. has a different meaning in other countries. In Australia, Bangladesh and Nigeria, this gesture is considered insulting (Kohl). In South Asian countries, gesturing to someone to accompany him to where he or she is supposed to sit down should not be made using the index finger in order to point to the location. This gesture is applicable only to animals. The polite way to beckon individuals to their seats is to bow slightly and with the back of your hand, indicate the place which you wish them to sit (Goman). Hailing a taxi cab could get different results depending on how you raise the palm of your hand. When you hail a cab with the palm of your hand raised, Asian cab drivers will interpret it as an aggressive gesture. In order to hail a cab properly and politely, the back of the hand must be held at the thigh level (Jones 4). Another source of difference in interpretation is the use of fingers (Stolte). Generally, when one hold up two fingers in the air, this means two. While this may seem a universally true interpretation, it means differently in other countries. Germany and France interprets the holding up of two fingers as three; therefore, tourists who order beer using this gesture will always get three beers at a bar in Germany. In both countries, counting always starts with the thumb. Hence, when Americans and Canadian use two fingers to count (also to say Peace!), the Germans and French will count two by using their thumb and a finger (also interpreted as a gun). Australians indicate time to drink up by folding three fingers of the hand against the palm, leaving the thumb and little finger sticking straight up and out. The same gesture means six in China (Jones 4). Another source of confusion is how to use the fingers and hand to call the attention of someone (Moran, Harris, and Moran 64). For instance, calling the attention of a waiter or waitress is done differently in different countries. In North America, all you need to do is to say Miss, or Sir, raise a finger or tilt your head to one side to call attention. Snapping ones fingers is considered impolite and disrespectful. In China and other Asian countries, the same task is accomplished by clinking a cup or a glass with your ring or a spoon. In Middle Eastern countries, clapping hands achieves the same result. In Japan, calling attention requires extending your arm slightly upward, palm down, and fluttering your fingers. In Spain and Latin America, people extend their hands, palm down, and then rapidly open and close their fingers (Jones 4). Handedness is also a source of confusion. All Muslim countries and some Asian nations consider the left hand as dirty and sinister, hence, using the left hand in receiving or presenting objects is frowned upon. The left hands use is for toileting and hygiene and is hence considered filthy (Jones 4). When taking food or other materials, one must always use the right hand (Goman). In Muslim countries, meals are communal gatherings and people scoop ones food with flat bread from a bowl found on the center. People sharing the food always use their right hand in scooping viand from the bowl. In Asian countries, using the right hand is the polite way of interacting with others. It is so because the right hand is the more dominant and adroit hand. When presenting or receiving a gift or any material however, Asians generally use both hands to signal ones appreciation for the goodwill (Jones 2). Shaking hands does not have a universal meaning or interpretation. In fact, the shaking of hands is particularly Western (Kohl). When dealing with other cultures, the rule is to never assume that the person in front of you or beside you is willing to shake your hand. Another rule especially true in Islamic countries is to never shake a womans hand. Another rule is not to shake someones hand unless that person extends his or her hand first (Goman). This is why some Americans who offer to shake someones hand upon first meetings are considered invasive and arrogant (Early and Ang 21). There are also ways in which shaking hands must be performed. For women in Asian countries, shaking hands means touching each others hands in a gentle manner. Even among men, the bone-crushing grip that is used by Western men when they shake hands is frowned upon in Eastern countries and is considered aggressive. Men of the Middle East and Asia simply brush their palms, which most Western men may find effe minate but for the former constitutes superior breeding and proper manners (Jones 3). A pat in the head or in the back is not similarly interpreted in different countries. In the US, patting the head of a small child is interpreted as affectionate. This gesture would be frowned upon in Malaysia and other Islamic countries because they believe that the head, being the source of spiritual and intellectual powers of a person, should be untouched (Early and Ang 22). Moreover, how Americans slap each other in the back to express camaraderie, delight, or to offer congratulations is not proper in Asian countries. This gesture would be considered aggressive and improper (Jones 5). Gestures that use the foot also hold different meanings for different cultures. In most cultures, such as in Asia and the Middle East, the sole of the foot is filthy and should be covered. Exposing the soles of the feet is disrespectful and insulting. Arabs usually direct the sole of the shoe toward the floor and do not do the typical leg-crossing of Western men where the calf or ankle of the crossing leg rests on the thigh of the other leg (Goman). In addition, among the people of a Laos tribe, when a woman taps the tap of a gentlemans foot with hers, she is indicating her desire to have sexual intercourse (Jones 5). Eye Contact Eye contact is very important in the West. Avoiding direct eye contact as seen in the movies is one way of detecting whether someone is lying (Goman). Hence, in Western culture, anybody who fails to maintain good eye contact is considered suspicious (Kohl). Other interpretations of people who avoid direct eye contact are unfriendly, insecure, untrustworthy, inattentive, and impersonal (Moran, Harris, and Moran 63). Conversely, avoiding ones gaze is a gesture of respect and of knowing ones place in Asian culture (Jones 3). For instance, Japanese children are socialized in school to focus their gaze on someones tie knot or an Adams apple. In China and in Japan, adults lower their gaze when speaking to a superior as a gesture of respect. In Latin American cultures and some Africa cultures, such as Nigeria, prolonged eye contact from one individual of lower status is considered disrespectful (Moran, Harris, and Moran 63). Staring is considered very rude in America while staring in China and other countries just indicates mere curiosity over someone and is not considered impolite or disrespectful (Kohl). In England, people are taught to pay strict attention to a speaker, to listen carefully, and to blink ones eyes to indicate comprehension or that one is listening. Americans signal interest and comprehension by bobbing their heads or grunting. A widening of the eyes can also be interpreted differently. For example, take the case of an American and a Chinese discussing the terms of a proposed contract. Regardless of the language in which the transaction is carried out, the U.S. may interpret a Chinese persons widened eyes as an expression of astonishment instead of its true meaning of politely suppressed Asian anger. (Moran, Harris, and Moran 64). Touch Recent studies reveal differences across cultures on the amount of touch applied and whether touch is displayed in public or in private (Early and Ang 78). For instance, in Tonga, when couples or families are parted, they weave their arms around one another, pray, and chant. Europeans usually lock in an embrace or share a passionate kiss publicly. Koreans do not use touch publicly. Moreover, it is customary for Austrian men to kiss the hand of a lady recently met in a restaurant and to go far as walk her to her table. While some cultures may find this disturbing or offensive, this is considered polite manners in Austria (Kohl). Interpreting Gestures One of the most significant studies on gestural difference across cultures was conducted by Dane Archer in 1997. Basing his data on a documentary made on people from various parts of the globe taking U.S. English as a Second Language, he provided a detailed inventory of some of the most commonly misinterpreted gestures from cultures all over the world. While errors are almost always forgiven, misinterpretation of gestures often leads to irritation, annoyance, and frustration among persons communicating. Below is an inventory of the gestural differences (Archer 81): 1. Good-Bye = Come Here (Japan) 2. Good Luck (U.S.) = Screw You (Iran) 3. Good Luck (U.S.) = Boyfriend (Japan) 4. Screw You (U.S.) = I Dont Believe You (Uruguay) 5. Im Angry (Nepal) = You Are Afraid (Mexico) 6. OK (U.S.) = Money (Japan) 7. OK (U.S.) = Sex (Mexico) 8. OK (U.S.) = Homosexual (Ethiopia) 9. Killed/Dead (U.S. throat slash) = Lost a Job (Japan) 10. Homosexual (U.S.) = Henpecked (Mexico) Conclusion Globalization has also meant respect, tolerance, and understanding of diverse cultures. The use of body language, hands, eyes, feet, fingers, and touch mean something to one culture and differently to another. As communication becomes increasingly intercultural, the need to be informed of how nonverbal communication across cultures vary enable us to be careful of what we say, how we use gestures, and how we apply nonverbal cues in order to get our message across effectively.

Essay --

DNA isolation and amplification of ansA1 and ansA3 genes Good quality genomic DNA was isolated (Sambrook, et al 2002) and bothansA1 and ansA3 genes were amplified by PCR. Clear bands of both the genes showing a size of 1kb were observed under UV transilluminator after agarose gel electrophoresis (Fig 1). Figure 1: PCR amplification of both ansA1 and ansA3 genes: Lane (A): Step up 100bp marker, Lane (B): amplification of ansA1 gene, Lane (C): amplification of ansA3 gene Overexpression and purification The overexpressed recombinant proteins (rBliAI and rBliAIII) of B. licheniformis MTCC 429 were purified to homogeneity by affinity chromatography using Ni-NTA column. Overexpression of recombinant His-tagged asparaginase into E.coli leads to improvement in yield and affinity purification process of enzyme (Enriquez et al, 2012). The fractions showing presence of protein with the help of Bradford’s reagent were pooled together. The pooled protein solution was dialyzed against the same buffer and was checked for the L-asparaginase activity. The purified protein after SDS PAGE or Native PAGE showed a single band (Fig 2) illustrating its homogeneous nature. The molecular weight of the subunits of ansA1 and ansA3was found to be approximately 37kD after SDS PAGE analysis. SDS PAGE analysis of recombinant L-asparaginase from Pyrococcus furiosus displayed single 37kDa band (Bansal et al, 2010). In another study, recombinant ansA from Rhizobium etliin SDS-PAGE showed the presence of a single polypeptide chain of 47kDa (Enriquez et al, 2012).Native PAGE analysis showed the molecular weight of the purified protein as ~74 kDa. This study confirmed that the ansA1 and ansA3 enzymes from Bacillus licheniformis MTCC 429 are a homodimer in... ...8 1.50x106 Roth et al, 2013 Table Comparative kinetic parameters for hydrolysis of L-asparagine The kinetic properties of recombinant BliA were investigated and the parameters like kcat and kcat/Km were also studied with L-asparagine as a substrate. Comparative studies of the rBliA with asparaginases from various microbial sources showed that rBliA has lower Km value for L-asparagine (Table 2) confirming thatrBliA hasbetter affinity towards its substrate than other reported (Table) microbial sources. The catalytic constant (kcat) of rBliA was only 1.5 times higher than the L-asparaginase from E.coli and catalytic constant of rErAII was 1.35 times higher than rBliA. The absolute value of kcat/Km suggests the catalytic efficiency of the enzyme (Price and Nairn, 2009). kcat/Km of the rBliAIII was 5.4x 104 which is lower than the asparaginases from E.coli and Erwinia. Essay -- DNA isolation and amplification of ansA1 and ansA3 genes Good quality genomic DNA was isolated (Sambrook, et al 2002) and bothansA1 and ansA3 genes were amplified by PCR. Clear bands of both the genes showing a size of 1kb were observed under UV transilluminator after agarose gel electrophoresis (Fig 1). Figure 1: PCR amplification of both ansA1 and ansA3 genes: Lane (A): Step up 100bp marker, Lane (B): amplification of ansA1 gene, Lane (C): amplification of ansA3 gene Overexpression and purification The overexpressed recombinant proteins (rBliAI and rBliAIII) of B. licheniformis MTCC 429 were purified to homogeneity by affinity chromatography using Ni-NTA column. Overexpression of recombinant His-tagged asparaginase into E.coli leads to improvement in yield and affinity purification process of enzyme (Enriquez et al, 2012). The fractions showing presence of protein with the help of Bradford’s reagent were pooled together. The pooled protein solution was dialyzed against the same buffer and was checked for the L-asparaginase activity. The purified protein after SDS PAGE or Native PAGE showed a single band (Fig 2) illustrating its homogeneous nature. The molecular weight of the subunits of ansA1 and ansA3was found to be approximately 37kD after SDS PAGE analysis. SDS PAGE analysis of recombinant L-asparaginase from Pyrococcus furiosus displayed single 37kDa band (Bansal et al, 2010). In another study, recombinant ansA from Rhizobium etliin SDS-PAGE showed the presence of a single polypeptide chain of 47kDa (Enriquez et al, 2012).Native PAGE analysis showed the molecular weight of the purified protein as ~74 kDa. This study confirmed that the ansA1 and ansA3 enzymes from Bacillus licheniformis MTCC 429 are a homodimer in... ...8 1.50x106 Roth et al, 2013 Table Comparative kinetic parameters for hydrolysis of L-asparagine The kinetic properties of recombinant BliA were investigated and the parameters like kcat and kcat/Km were also studied with L-asparagine as a substrate. Comparative studies of the rBliA with asparaginases from various microbial sources showed that rBliA has lower Km value for L-asparagine (Table 2) confirming thatrBliA hasbetter affinity towards its substrate than other reported (Table) microbial sources. The catalytic constant (kcat) of rBliA was only 1.5 times higher than the L-asparaginase from E.coli and catalytic constant of rErAII was 1.35 times higher than rBliA. The absolute value of kcat/Km suggests the catalytic efficiency of the enzyme (Price and Nairn, 2009). kcat/Km of the rBliAIII was 5.4x 104 which is lower than the asparaginases from E.coli and Erwinia.